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Biochem. Soc. Symp. (2002) 69, (135–142) (Printed in Great Britain)
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Chapter 11
Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling |
| Elena M. Comelli*‡†§, Margarida Amado*‡§, Steven R. Head*‡§ and James C. Paulson*‡§1 |
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| *Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, U.S.A., ‡Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, U.S.A., †Nestlé Research Centre, Lausanne, Switzerland, and §the DNA Array Core Facility, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, U.S.A.
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1To whom correspondence should be addressed (e-mail jpaulson@scripps.edu).
Abstract:
The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.
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